Year: 2021 | Month: September | Volume 14 | Issue 3
Direct In Vitro Shoot Regeneration from Spindle Leaf Roll Segments of Sugarcane
Jashandeep Singh Gill
Jagdeep Singh Sandhu
DOI:10.30954/0974-1712.03.2021.4
Abstract:
The spindle leaf roll segments of sugarcane cultivar Co 238 were cultured in vitro for shoot regeneration. Cultured spindles exhibited unwhorling after 5 to 10 days of incubation followed by direct shoot regeneration without callus phase. Shoot initiation was observed in a cluster of 2-3 shoots after the third week of incubation. Data was recorded for the number of shoots, shoot length, rooting frequency, and root length. Maximum of 7.68 ± 1.26 shoots with mean shoot length of 8.13 ± 1.87 cm, 100 % root induction frequency, and mean root length of 3.89 cm were recorded. This study demonstrates the development of a reliable method for sugarcane improvement and can be used for micropropagation and genetic engineering of Co 238 cultivar.
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