Year: 2018 | Month: April | Volume 8 | Issue 1
HBP Concentration Affects in vitro Acrosome Reaction and Conception Rate in Murrah Buffalo Bulls: Interrelationship among Sperm function tests
Abstract:
The aim of this study was to identify relationship between heparin binding proteins (HBP) concentration and in vitro acrosome reaction / conception rate / sperm function tests in murrah buffalo bulls. Frozen-thawed semen of 31 bulls was analyzed for HBP concentration, in vitro acrosome reaction, acrosome integrity, DNA integrity and lipid peroxidation. Conception rate of 10 bulls (21-30) was also obtained. Relationship between HBP and in vitro acrosome reaction / conception rate was evaluated. Inter-relationship among sperm function tests was also observed. A considerable variation in motility (PTM), DNA integrity, acrosome damage (fully damaged: FDA, partially damaged: PDA), in vitro capacitation / acrosome reaction and LPO was observed among 31 tested buffalo bulls after thawing during the present study. A significant positive correlation was observed among various sperm parameters. Thirty one bulls were divided into G-1 (> 40 % AR) and G-II (≤ 40 % AR). There was no difference in HBP concentration, PTM and DNA damaged sperms among the two groups. Percentage of spermatozoa with partially, fully damaged and acrosome reacted spermatozoa was significantly (p ≤ 0.05) higher in G- I as compared to G-II. MDA level (μmoles / 109 spermatozoa) was non-significantly (p ≥ 0.05) higher in G-II as compared to G-I. Ten bulls (22-31) were divided into two groups; G-I (> 35 % conception rate) and G-II (< 35 % conception rate). Concentration of HBPs was significantly (p ≤ 0.05) higher in G-I as compared to G-II. Percentage of acrosome reacted spermatozoa was also significantly (P ≤0.05) higher in G-I than G-II. Therefore, HBP concentration in spermatozoa may be useful in predicting the fertility of buffalo bull semen.
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