Year: 2017 | Month: August | Volume 7 | Issue 4
Genotypic Study of Verocytotoxic E. coli in Cattle by Multiplex Polymerase Chain Reaction
Abstract:
E. coli is the most commonly observed gastrointestinal flora of animals and environmental contaminant considered as important food borne pathogen causing serious complications in man and animals. The study was undertaken to detection of virulence gene using polymerase chain reaction (PCR) in cattle. In present study, a total of 160 samples were processed for isolation of verocytotoxic E. coli (VTEC). All samples were positive for Escherichia coli. Out of 160 Escherichia coli, 14 samples were found positive for VTEC. Out of 14 VTEC, 6 were found to be positive for stx1 gene (180 bp), 5 were stx2 (255 bp) and 3 were for stx1, stx2 and hlyA gene.
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